Study of Chondrogenic Effects of Chondrocytes Cocultured With Murine Bone Marrow-Derived Mesenchymal Stem Cells

Authors

  • Baghban Eslami Nezhad, Mohammad Reza
  • Taghi Yar, Leyla Stem Cells Department, Royan Institute, Tehran, Iran.
Abstract:

Purpose: Co-culture systems of marrow derived mesenchymal stem cells (mMSCs) with mature chondrocytes have theoretically been considered as a putative way of MSCs chondrogenic differentiation. MSCs differentiated in this system could be used for transplantation purpose without of any need to their purification since the cells with which MSCs are co cultured are native cartilage cells. Despite of the importance of this system, the data is very rare in this context which is subject of the present study.  Materials and Methods: The mesenchymal stem cells were isolated from the bone marrow of 4-6 weeks old NMRI mice by low-density primary culture system and expanded by performing two successive subcultures. Chondrocytes were obtained from rat costal cartilage and cultivated in 25-cm2 flasks for a period of two weeks. To prepare co culture systems, passaged-2 mesenchymal cells and primary-cultured chondrocytes were used. In present study, the different cell ratios of MSCs and chondrocytes including 1/1, 1/2 and 2/1 were prepared and cultivated in a micro mass culture systems in a medium lacking the factors known to induce cartilage differentiation. In the end of cultivation periods (21 days), the groups were examined by toluidine blue staining technique and RT-PCR analysis for murine cartilage differentiation. Results: Spindly–shaped murine mesenchymal cells were dominated the culture by second subculture. In the case of rat chondrocyte, these cells were reached to confluency 2 weeks after culture initiation. Co culture results indicated that in the group of 1/2 ratio (when the number of chondrocyte were as twice as MSCs), the abundant of metachromatic matrix containing collagen II, X and aggreacan were produced whereas in other groups this matrix were rarely observed. Conclusion: Chondrocyte co cultured with mesenchymal stem cells seemed to practically produce chondrogenic signal enough to promote intensive MSCs cartilage differentiation. In this system the ratio of the co cultured cells is important.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells

In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...

full text

Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions

Objective(s):Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet.  The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages. Material...

full text

Subchondral bone influences chondrogenic differentiation and collagen production of human bone marrow-derived mesenchymal stem cells and articular chondrocytes

INTRODUCTION Osteoarthritis (OA) is characterized by an imbalance in cartilage and underlying subchondral bone homeostasis. We hypothesized that signals from the subchondral bone may modulate production of matrix components, alter chondrogenic differentiation potential of cocultured bone marrow-derived mesenchymal stem cells (BMSC) and induce a phenotypic shift in differentiated OA chondrocytes...

full text

Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats

The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of th...

full text

Matrigel Enhances in vitro Bone Differentiation of Human Marrow-derived Mesenchymal Stem Cells

Objective(s) The use of co-culture cells as well as extra cellular matrix are among those strategies that have been employed to direct mesenchymal stem cell (MSC) bone differentiation in culture. In this regard, there is no study considering the effects of Matrigel on mesenchymal stem cell (MSC) in vitro bone differentiation. This was the subject of the present study. Materials and Methods ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 4  issue None

pages  215- 224

publication date 2006-10

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023